Evaluate the expression of four virulence factors (fedA, faeG, eae and toxA from Escherichia coli) by using qPCR. toxA, coding for the heat-labile enterotoxin (LT); eae, coding for the intimine; fedA, coding for the F18 fimbriae and faeG, coding for the F4 fimbriae. Post-weaning diarrhea is an important enteric disease that usually occurs shortly after weaning and is characterized by watery diarrhea, dehydration, loss of body weight, and the death of infected pigs. This diarrhea is responsible for important economic losses due to mortality, morbidity, decreased growth rate and cost of medication. Escherichia coli is one of the most important causes of the increasing worldwide outbreaks of post-weaning diarrhea, causing sudden death or severe diarrhoea. The PCR identification of virulence factors of E. coli from fecal samples is normally used for the diagnosis of pathogenic isolates.
The Real Time PCR detection Kit Weaned Pig Diarrhoea GeneExpres RT-qPCR Panel (fedA, faeG, eae, toxA and Stx2 from Escherichia coli) comprises a series of specific targeted reagents designed for Escherichia coli Stx2, toxA, eae, faeG and fedA detection by using qPCR
The Real Time PCR detection Kit Weaned Pig Diarrhoea GeneExpres RT-qPCR Panel (Escherichia coli Stx2, toxA, eae, faeG, fedA, pHSTA and HSTB) comprises a series of specific targeted reagents designed for Escherichia coli Stx2, toxA, eae, faeG, fedA, pHSTA and HSTB detection by using qPCR
The Bovine respiratory multiplex (BRM)-Bacteria qPCR kit allows to detect in bovine sample Mycoplasma bovis (M. bovis), Mannheimia haemolytica (M. haemolytica), Pasteurella multocida (P. multocida) and Histophilus somni (H. somni) pathogens in the same reaction with a high sensitivity.