Real Time PCR Detection Kit Weaned Pig Diarrhoea GeneExpres RT-qPCR Panel 7 targets

The Real Time PCR detection Kit Weaned Pig Diarrhoea GeneExpres RT-qPCR Panel (Escherichia coli Stx2, toxA, eae, faeG, fedA, pHSTA and HSTB) comprises a series of specific targeted reagents designed for Escherichia coli Stx2, toxA, eae, faeG, fedA, pHSTA and HSTB detection by using qPCR.

GENERAL INFORMATION:Post weaning diarrhoea is an important enteric disease that usually occurs shortly after weaning. It is characterized by watery diarrhoea, dehydration, loss of body weight and death of infected pigs. This diarrhoea is responsible for important economic losses due to mortality, morbidity, decreased growth rate and cost of medication. Escherichia coli is one of the main causes of the increasing number of outbreaks worldwide of post weaning diarrhoea, causing sudden death or severe diarrhoea. The PCR identification of virulence factors of E. coli from faecal samples is used for diagnosis of pathogenic isolates. This kit allows evaluation of the expression of virulence factors such as toxA, coding for the heat-labile enterotoxin (LT); eae, coding for the intimine; fedA, coding for the F18 fimbriae; faeG, coding for the F4 fimbriae; Stx2, coding for the Shiga toxin 2; pHSTA, coding for the porcine Heat-stable toxin A; and HSTB, coding for the Heat-stable toxin B.

KIT CHARACTERISTICS:
- Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
- Resuspension buffer
- DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control