Real Time PCR Detection Kit Weaned Pig Diarrhoea GeneExpres RT-qPCR Panel 5 targets

The Real Time PCR detection Kit Weaned Pig Diarrhoea GeneExpres RT-qPCR Panel (fedA, faeG, eae, toxA and Stx2 from Escherichia coli) comprises a series of specific targeted reagents designed for Escherichia coli Stx2, toxA, eae, faeG and fedA detection by using qPCR.

GENERAL INFORMATION:Post weaning diarrhoea is an important enteric disease that usually occurs shortly after weaning and is characterized by watery diarrhoea, dehydration, loss of body weight and death of infected pigs. This diarrhoea is responsible for important economic losses due to mortality, morbidity, decreased growth rate and cost of medication. Escherichia coli is one of the most important causes of the increasing worldwide outbreaks of post weaning diarrhoea, causing sudden death or severe diarrhoea. The PCR identification of virulence factors of E. coli from faecal samples is normally used for diagnosis of pathogenic isolates. This kit allows evaluation of the expression of some of this virulence factors as toxA, coding for the heat-labile enterotoxin (LT); eae, coding for the intimine; fedA, coding for the F18 fimbriae; faeG, coding for the F4 fimbriae; and Stx2, coding for the Shiga toxin 2.

KIT CHARACTERISTICS:
- Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
- Resuspension buffer
- DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control