The expression of six virulence factors (faeG, eae, toxA from Escherichia coli, tcdA from Clostridium difficile and cpa, cpb2 from Clostridium perfringens) by using qPCR. Of all the diseases in the sucking piglet, diarrhea is the most common. Due to the limited body reserves of the newborn piglet, this will soon lead to a severe condition and the piglet may die within hours. The Kit allows the evaluation mRNA expression of six virulence factors from different organisms related to neonatal pig diarrhea. The E. coli genes: toxA, coding for the heat-labile enterotoxin (LT); eae, coding for the intimine and faeG, coding for the F4 fimbriae. The gene tcdA coding for the C. difficile toxin A. Genes cpa and cpb2 coding for the alpha and beta 2 toxins from C. perfringens.
The Bacterial swine diarrhea designed for the Detection test kit of Brachyspira hyodysenteriae, Brachyspira pilosicoli and Lawsonia intracellularis by using qPCR. Enteric bacterial infections are among the most common and economically significant diseases affecting swine production worldwide. This panel was designed to detect three microorganisms related to bacterial swine diarrhea. Brachyspira hyodysenteriae (formerly Treponema hyodysenteriae and Serpulina hyodysenteriae), Brachyspira pilosicoli (formerly Serpulina pilosicoli), Lawsonia intracellularis.
Virulence fimbrial genes from Escherichia coli F5, F17 and F41 detection test kit by using qPCR. Most E. coli strains are harmless, but some serotypes are pathogenic and can cause serious food poisoning in animals and humans. F5, F17 and F41 are relevant fimbrial genes related to enterotoxigenic and uropathogenic strains of E. coli.
Detection test kit of 10 different virulence genes from Escherichia coli by using qPCR. Some E. coli are pathogenic, meaning they can cause illness, either diarrhea or illness outside of the intestinal tract. The types of E. coli that can cause diarrhea can be transmitted through contaminated water or food, or through contact with animals or persons. Virulence genes detected include the Aerobactin synthase (iucC), Ligand-gated channel protein (fyuA), Ferric enterobactin receptor (iroN) and Aerobaction siderophore ferric receptor (iutA), all of them involved in iron transport under iron-stressed conditions; Temperature-sensitive hemagglutinin tsh autotransporter (tsh) can act both as an adhesin and as a serine protease; Colicin-V immunity protein (cvi) protects a cell against colicin V; Protein iss (iss) increases serum survival and confers group II surface exclusion; Chaperone protein (fimC) required for the biogenesis of type 1 fimbriae; ompT protein (ompT) aspartyl protease found on the outer membrane and Hemolysin F (hlyF) fatty-acyl-CoA reductase activity.