Real Time PCR Detection Kit Neonatal Pig Diarrhoea GeneExpres Panel 8 targets

The Real Time PCR detection Kit Neonatal Pig Diarrhoea GeneExpres Panel (Escherichia coli toxA, eae, faeG, pHSTA, HSTB and Clostridium perfringens Beta, Epsilon, Beta2 toxins) comprises a series of specific targeted reagents designed for Escherichia coli toxA, eae, faeG, pHSTA, HSTB and Clostridium perfringens Beta, Epsilon, Beta2 toxins detection by using qPCR.

GENERAL INFORMATION:Of all the diseases in the sucking piglet, diarrhoea is the most common and probably the most important. The consequences of profuse, watery diarrhoea will be a rapid loss of water, electrolytes, and nutrients. Due to the limited body reserves of the new-born piglet, this will soon lead to a severe condition and the piglet may die within hours. The NPDiarrhoea-Panel-8 allows to evaluate the mRNA expression of eight virulence factors from different organisms related with neonatal pig diarrhoea. The Clostridium perfringens genes cpb, coding for the Beta toxin; etx, coding for the Epsilon toxin and cpb2, coding for the Beta 2 toxin. Additionally, faeG, coding for the F4 fimbriae; toxA, coding for the heat-labile enterotoxin (LT); eae, coding for the intimine, porcine Heat-stable toxin A and Heat-stable toxin B from Escherichia coli.

KIT CHARACTERISTICS:
- Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
- Resuspension buffer
- DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control