The Real Time PCR detection Kit Weaned Pig Diarrhoea GeneExpres RT-qPCR Panel (fedA, faeG, eae, toxA and Stx2 from Escherichia coli) comprises a series of specific targeted reagents designed for Escherichia coli Stx2, toxA, eae, faeG and fedA detection by using qPCR
The Real Time PCR detection Kit Weaned Pig Diarrhoea GeneExpres RT-qPCR Panel (Escherichia coli Stx2, toxA, eae, faeG, fedA, pHSTA and HSTB) comprises a series of specific targeted reagents designed for Escherichia coli Stx2, toxA, eae, faeG, fedA, pHSTA and HSTB detection by using qPCR
The expression of six virulence factors (faeG, eae, toxA from Escherichia coli, tcdA from Clostridium difficile and cpa, cpb2 from Clostridium perfringens) by using qPCR. Of all the diseases in the sucking piglet, diarrhea is the most common. Due to the limited body reserves of the newborn piglet, this will soon lead to a severe condition and the piglet may die within hours. The Kit allows the evaluation mRNA expression of six virulence factors from different organisms related to neonatal pig diarrhea. The E. coli genes: toxA, coding for the heat-labile enterotoxin (LT); eae, coding for the intimine and faeG, coding for the F4 fimbriae. The gene tcdA coding for the C. difficile toxin A. Genes cpa and cpb2 coding for the alpha and beta 2 toxins from C. perfringens.
The Bacterial swine diarrhea designed for the Detection test kit of Brachyspira hyodysenteriae, Brachyspira pilosicoli and Lawsonia intracellularis by using qPCR. Enteric bacterial infections are among the most common and economically significant diseases affecting swine production worldwide. This panel was designed to detect three microorganisms related to bacterial swine diarrhea. Brachyspira hyodysenteriae (formerly Treponema hyodysenteriae and Serpulina hyodysenteriae), Brachyspira pilosicoli (formerly Serpulina pilosicoli), Lawsonia intracellularis.
Evaluate the expression of four virulence factors (fedA, faeG, eae and toxA from Escherichia coli) by using qPCR. toxA, coding for the heat-labile enterotoxin (LT); eae, coding for the intimine; fedA, coding for the F18 fimbriae and faeG, coding for the F4 fimbriae. Post-weaning diarrhea is an important enteric disease that usually occurs shortly after weaning and is characterized by watery diarrhea, dehydration, loss of body weight, and the death of infected pigs. This diarrhea is responsible for important economic losses due to mortality, morbidity, decreased growth rate and cost of medication. Escherichia coli is one of the most important causes of the increasing worldwide outbreaks of post-weaning diarrhea, causing sudden death or severe diarrhoea. The PCR identification of virulence factors of E. coli from fecal samples is normally used for the diagnosis of pathogenic isolates.
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