Real Time PCR Detection Kit Bacterial Swine Diarrhoea Panel

Background:The Bacterial swine diarrhea Panel comprises a series of species-specific targeted reagents designed for the detection of Brachyspira hyodysenteriae, Brachyspira pilosicoli and Lawsonia intracellularis by using qPCR.
Enteric bacterial infections are among the most common and economically significant diseases affecting swine production worldwide. Clinical signs of these infections include diarrhea, reduced growth rate, weight loss, and death of preweaned, weanling, grower-finisher, young and adult age breeding animals. This panel was designed to detect three microorganisms related to bacterial swine diarrhea. Brachyspira hyodysenteriae (formerly Treponema hyodysenteriae and Serpulina hyodysenteriae) is a Gram-negative, motile, loosely coiled spirochete belonging to Brachyspiraceae family. This pathogenic bacteria colonizes the large intestine of pigs causing dysentery. Swine dysentery is easily spread by the fecal-oral route due to the ability of B. hyodysenteriae to survive several weeks in moist feces from infected pigs. Brachyspira pilosicoli (formerly Serpulina pilosicoli) is a Gram-negative, spiral-shaped, obligate anaerobe bacterium member of the Spirochaete family. It causes intestinal spirochaetosis in pigs. Infection of B. pilosicoli is acquired through the fecal-oral route. Once in the alimentary tract, the bacterium produces edema, hemorrhage, and infiltration by inflammatory cells. Lawsonia intracellularis is a Gram-negative, obligated intracellular bacterium belonging to Desulfovibrionaceae family. It causes intestinal hyperplasia mainly in pigs. The disease has two clinical manifestations in pigs: acute hemorrhagic diarrhea and sudden death of pigs close to market age often called proliferative hemorrhagic enteropathy, and chronic mild diarrhea with poor growth performance in grower-finisher pigs referred to as porcine intestinal adenomatosis.

Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control