DNA/RNA Extraction and Purification

PCR purification/Gel extraction: Efficient and fast method for the purification and/or concentration of high quality DNA fragments (70bp to 15kb) from PCR reactions, enzymatic restriction digestion or from agarose gels. Recovers up to 95% (PCR Clean-up) or up to 90% (Gel Extraction)

Plasmid purification: Efficient and fast method for the purification of high quality plasmid DNA from 1-6 ml of cultured bacterial cells. Eluted DNA is suitable for all common downstream applications including PCR, enzymatic restriction digestion, cloning and DNA sequencing

Genomic DNA purification: for purification and/or concentration from samples containing partial purified DNA

Total RNA isolation: for purification and/or concentration from samples containing partial purified RNA

Total RNA isolation: for Gram (+) positive and Gram (-) negative bacteria

Total RNA isolation: for fresh whole blood and cultured animal cells

Total RNA isolation: for plant tissue and cells

Total RNA isolation: for a wide variety of tissues and FFPE

Total RNA isolation: for yeast and a wide variety of fungus species

Purification of DNA and/or RNA: for DNA and RNA from cell-free media (serum, plasma, body fluids and the supernatant from viral infected cell cultures)

The LITE Genomic DNA Kit – Bacteria – provides an efficient and fast method for the purification of high quality genomic (and viral) DNA from Gram-positive and Gram-negative bacteria, suitable for all common downstream applications such as PCR, enzymatic restriction digestion, cloning, Southern blot analysis, etc.

The LITE Genomic DNA Kit – Blood & Cultured Cells – provides an efficient, fast and economic method for the purification and or concentration of high quality total DNA (including genomic DNA, mitochondrial DNA and viral DNA) from a variety of samples

The LITE Genomic DNA Kit – BroadRange- provides an efficient, fast and economic method for the purification of high quality total DNA (including genomic DNA, mitochondrial DNA and viral DNA) from a broad variety of samples.

The LITE Genomic DNA Kit – Plant – provides an efficient and fast method for the purification and or concentration of high quality total DNA (including genomic DNA, mitochondrial DNA, and chloroplast DNA) from plant tissue and cells.

The LITE Genomic DNA Kit – Tissue – provides an efficient and fast method for the purification and or concentration of high quality total DNA (including genomic DNA, mitochondrial DNA and viral DNA) from a variety of tissues, including tailsnips, liver, kidney, brain, adipose tissue, earpunches, insects, and FFPE.

The LITE PCR & Gel Band Purification Kit provides an efficient and fast method for the purification and or concentration of high quality DNA fragments (100bp to 10kb) from PCR reactions, enzymatic restriction digestion or from agarose gels.