GRS Total RNA Kit - Plant

Total RNA isolation: for plant tissue and cells
SKU: GK10.0100

Register or Log In in order to request a quote

Register Login

Products specifications
TechnologyqPCR / Real Time PCR/ PCR
Sizes100 preps/ kit
Total RNA isolation: for plant tissue and cells

The GRS Total RNA Kit – Plant – provides an efficient and fast method for the purification and or concentration of high quality total RNA (including mRNA, tRNA and rRNA) from plant tissue and cells. Optional DNAse treatment can be included in the protocol to remove undesired DNA residue. Eluted purified RNA is suitable for RT-PCR, One-step qRT-PCR, Northern Blotting, mRNA selection, cDNA synthesis, and primer extension.
Principle: Samples are ground in liquid nitrogen and subsequently filtered to remove debris. The buffer system is optimized to allow selective binding of RNA to the glass fiber matrix of the spin column. Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step.
The purified RNA is subsequently eluted with RNAse-Free Water. The entire procedure can be completed within 30 minutes with typical RNA yields of 5-30μg without the use of phenol extraction.
Sample : up to 100mg of fresh plant tissue, up to 25 mg of dry plant tissue
Expected Yield : 5-30μg total RNA from young leaf samples
Format : spin column (certified DNAse/RNAse-free)
Operation Time : within 30 minutes
Elution Volume : 50μl
Similar Products

GRS Total RNA Kit - Bacteria

Total RNA isolation: for Gram (+) positive and Gram (-) negative bacteria

GRS Total RNA Kit - Yeast & Fungus

Total RNA isolation: for yeast and a wide variety of fungus species

GRS Total RNA Kit - Tissue

Total RNA isolation: for a wide variety of tissues and FFPE

GRS Total RNA Kit - Blood & Cultured Cells

Total RNA isolation: for fresh whole blood and cultured animal cells

GRS Genomic DNA Kit - Plant

Genomic DNA purification: for Plant tissue and cells