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Products specifications
| Technology | qPCR / Real Time PCR/ PCR |
| Sizes | DVEV04413: 100 reactions - FAM, Cy5 DVEV04453: 50 reactions - FAM, Cy5 |
Description
Detection of the RNA of the the capsid protein gene of Black Queen Cell Virus (BQCV)
Background:Black queen cell virus (BQCV) is a non-enveloped single-stranded RNA virus and belongs to the Dicistroviridae family and to the genus Triatovirus. The virus is the most common cause of death in queen´s larvae and pupae after sealing their cells, especially of A. mellifera, A. florea and A. dorsata. Viral infection turn the cell wall´s and brood´s color into brown and black. The virus is transmitted through feeding of the larvae, from hive to hive and via Nosema apis as a parasitic vector. Infection of queens, young workers or adult bees could happen but without visible symptoms.
Description:ViroReal® Kit Black Queen Cell Virus is a real-time PCR for the qualitative detection of the RNA of the the capsid protein gene of Black Queen Cell Virus (BQCV). Our carefully designed primers and probes ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).
Product Features:
- Amplification and detection: RNA of capsid protein of Black Queen Cell Virus
- Transcription with thermostable MMLV Reverse Transcriptase
- Contains RNase Inhibitor to block RNA degradation
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix)
Background:Black queen cell virus (BQCV) is a non-enveloped single-stranded RNA virus and belongs to the Dicistroviridae family and to the genus Triatovirus. The virus is the most common cause of death in queen´s larvae and pupae after sealing their cells, especially of A. mellifera, A. florea and A. dorsata. Viral infection turn the cell wall´s and brood´s color into brown and black. The virus is transmitted through feeding of the larvae, from hive to hive and via Nosema apis as a parasitic vector. Infection of queens, young workers or adult bees could happen but without visible symptoms.
Description:ViroReal® Kit Black Queen Cell Virus is a real-time PCR for the qualitative detection of the RNA of the the capsid protein gene of Black Queen Cell Virus (BQCV). Our carefully designed primers and probes ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).
Product Features:
- Amplification and detection: RNA of capsid protein of Black Queen Cell Virus
- Transcription with thermostable MMLV Reverse Transcriptase
- Contains RNase Inhibitor to block RNA degradation
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix)
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