TSH rat ELISA

Background:Thyroid-stimulating hormone (also known as thyrotropin or TSH) is a glycoprotein produced by the anterior pituitary gland. Through its action on the thyroid gland, it plays a major role in maintaining normal circulating levels of the iodothyronines, T4 and T3. The production and secretion of TSH is controlled on the one side by negative feedback from circulating T4 and T3, and on the other side by the hypothalamic thyrotropin-releasing hormone (TRH). The TSH molecule is composed of two non-identical subunits, α and β, that are bound together in a noncovalent manner. Within a species, the TSH α subunit is structurally identical to the α subunits of related glycoprotein hormones (LH, FSH). The β subunits of the related hormones are structurally hormone-specific and therefore determine their unique biological activities. The mechanism controlling thyroid function in rats is exactly analogous to the mechanism operating in humans. This means that thyrotropin-releasing hormone stimulates the release of TSH from the pituitary gland as well as the serum concentrations of T4 and T3 influence the action of the pituitary gland. This similarity between rat and human thyroid physiology makes the rat a very useful model for evaluating the effects of new drugs on thyrometabolic status.

Description:
The test kit is a solid phase enzyme-linked immunosorbent assay (ELISA) in the microplate format, designed for the quantitative measurement of TSH in rat serum. The microplate is coated with a monoclonal antibody specific for TSH. Calibrators and samples are pipetted into the antibody-coated microplate. Afterwards, a polyclonal horseradish peroxidase-labeled antibody is added. During a 16-24 hours incubation at 2-8°C sandwich complexes consisting of the two antibodies and the rat TSH are formed. Non-reactive components are removed by a washing step. A chromogenic substrate, TMB (3,3',5,5'-tetramethylbenzidine), is added to all wells. During a 30 minutes incubation, the substrate is converted to a colored end product (blue) by the bound enzyme. Enzyme reaction is stopped by dispensing hydrochloric acid as stop solution (change from blue to yellow). The color intensity is directly proportional to the concentration of rat TSH present in the sample. The Optical Density (OD) of the color solution is measured with a microplate reader at 450 nm.

Product features:
The kit contains reagents for 96 determinations;
Microtiter plate consisting of 12x8 (breakapart);
Microtiter plate reader at 450 nm;
Analytical sensitivity: 0,081 ng/mL.