GRO/CINC-1 rat ELISA

Background: Cytokine-induced neutrophil chemoattractant-1 (CINC-1) was originally purified from media conditioned by IL-1β stimulated rat kidney epithelioid cells (NRK-52E). Watanabe’s group at Toyama Medical and Pharmaceutical University identified amino acid sequence that encodes for rat CINC-1 in 1989. CINC-1 is a member of the alpha (CXC) subfamily of chemokines. Three additional rat CXC chemokines (CINC-2α, CINC-2β, CINC-3/MIP-2) have been identified. The protein sequence of CINC-1 is 63 - 67% identical to that of CINC-2α, CINC-2β, CINC-3/MIP-2. In addition, GROα, GROβ and GROγ is sharing 68%, 71% and 69%, identity with CINC-1. This has been suggested that CINCs are the rat counterpart of human GROs.

Description:
This kit is a solid phase sandwich ELISA using 2 kinds of high specific antibodies. Tetra Methyl Benzidine (TMB) is used as coloring agent (Chromogen). The strength of coloring is in proportion to the quantities of Rat GRO/ CINC-1.

Product features:
The kit contains reagents for 96 determinations;
Microtiter plate consisting of 12x8;
Microtiter plate reader at 450 nm;
Analytical sensitivity: 0,35 pg/mL