Estradiol rat ELISA

>Background:Estradiol (E2 or 17β-estradiol) is an estrogenic hormone produced by the ovaries and in smaller amounts by the adrenal cortex and testes. It is the most potent female sexual hormone and is essential for maintaining normal female functions. During the oestrous cycle which can be divided into four phases (proestrus, estrus, metestrus, diestrus) estradiol concentrations rise gradually from metestrus to proestrus and fell to barely detectable levels in estrus. The maximum concentration is reached around mid-day of proestrus. Apart from its effects on sexual characteristics it has important influence on the growth and development of the brain. In female rodents, the determination of estradiol is a useful marker in evaluating and monitoring the state of the reproductive functions and pregnancy as well.

Description:
The Estradiol rat ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with an anti-Estradiol antibody. An unknown amount of estradiol present in the sample competes with an Estradiol-horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is inversely proportional to the concentration of Estradiol in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of free Estradiol in the sample.

Product features:
The kit contains reagents for 96 determinations;
Microtiter plate consisting of 12x8 (breakapart);
Microtiter plate reader at 450 nm;
Assay dynamic range: 2,5 – 1280 pg/mL.