The Real Time PCR detection Kit Porcine Full Screening Pathogens DNA Panel (Porcine circovirus type 2, Metamycoplasma hyosynoviae, Haemophilus parasuis, Mycoplasma hyorhinis, Pasteurella multocida, Mycoplasma hyopneumoniae, Porcine circovirus type 3, Actinobacillus pleuropneumoniae Apx IV toxin, Streptococcus suis, Bordetella bronchiseptica and Leptospira “pathogenic) comprises a series of specific targeted reagents designed for Porcine circovirus type 2, Mycoplasma hyosynoviae, Haemophilus parasuis, Mycoplasma hyorhinis, Pasteurella multocida, Mycoplasma hyopneumoniae, Porcine circovirus type 3, Actinobacillus pleuropneumoniae Apx IV toxin, Streptococcus suis, Bordetella bronchiseptica and Leptospira “pathogenic” detection by using qPCR.