Rat DMP1 Assay Kit

Background:Dentin Matrix Protein 1(DMP1) is a constitutive protein in extracellular matrix of osteocyte and it belongs to Small Integrin-Binding Ligand, N-linked Glycoprotein (SIBLING) family. It is synthesized as a full-length 105kDa proprotein and it is divided into 37kDa at N terminal side and 57kDa at C terminal side in the secretion process. It has a lot of acidic domains and it binds with calcium because of the feature that become negative charged within tissues and it has an important role for bone mineralization. DMP1 expresses in osteocyte while other constitutive proteins in extracellular matrix such as Osteopontin, Osteocalcin and Bone sialoprotein express in osteoblast cells. Osteocyte which is a main constituent cell of bone regulates activities of osteoclastic cells and osteoblast cells and it adjusts bone remodeling process. It also acts as an endocrine cell which secrets FGF-23. DMP1 in blood is considered as a candidate of biomarker which reflects activities of osteocyte and it is also suggested to become useful information for research of bone metabolism and phosphate metabolism. The concentration of DMP1 in rat blood can be quantitatively measured by this ELISA kit.

Description:
This kit is a solid phase sandwich ELISA using 2 kinds of highly specific antibodies. Tetra Methyl Benzidine (TMB) is used as a coloring agent (Chromogen). The strength of coloring is proportional to the quantities of Rat DMP1.

Product features:
The kit contains reagents for 96 determinations;
Microtiter plate consisting of 12x8;
Microtiter plate reader at 450 nm;
Analytical sensitivity: 4,04 pg/mL