Histamine ELISA

>Background:Histamine is a biogenic amine and neurotransmitter and is formed from the amino acid L-histidine. It is synthesized and stored in mast cells and basophils until it is released upon appropriate stimulation and finally degraded by diamine oxidase and N-methyltransferase. Histamine is involved in many mechanisms through its release, such as immunological, physiological, and inflammatory mechanisms, as well as smooth muscle contraction, vasodilation, and increased vascular permeability. These mechanisms may result in various clinical pathologies such as diabetes, migraine, and stress, or may also affect sleep/wake states. Histamine has been widely described as a mediator of allergic reactions, such as hay fever, skin eczema, asthma, and anaphylactic reactions. Thus, histamine testing in food intolerances or other allergic reactions can provide an indication of the severity of the intolerance or allergy. If the histamine value is outside the reference range, the results should be clarified with a therapist or physician to discuss further action. Therapeutic consequences should never be based on laboratory results alone, even if these results are assessed in accordance with the quality criteria of the method. Any laboratory result is only a part of the total clinical picture of the patient. Only in cases where the laboratory results are in an acceptable agreement with the overall clinical picture of the patient, it can be used for therapeutic consequences.

Description:
In the first part of the procedure, histamine is quantitatively acylated to N-acyl histamine. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples compete with the solid phase bound analytes for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-goat IgG-peroxidase conjugate using TMB as a substrate resulting in a colour reaction. The reaction is monitored at a wavelength of 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations. Manual processing of the ELISA is recommended. The use of automatic laboratory equipment is the responsibility of the user. This in-vitro diagnostic is for professional use only.

Product features:
The kit contains reagents for 96 determinations;
Microtiter plate consisting of 12x8;
Microtiter plate reader at 450 nm;
Analytical sensitivity: plasma: 0.18 ng/ml; urine: 0.22 ng/ml;
IVD.