Serotonin ELISA

>Background:Serotonin (5-hydroxytryptamine) is an intermediate product of tryptophan metabolism, a well-studied neurotransmitter, and may also act as a peripheral hormone. Synthesis occurs mainly in enterochromaffin cells (ec-cells) of the gastrointestinal tract and in neurons. It is present in high concentrations in ec-cells of the intestine, serotonergic neurons of the brain, and platelets. Serotonin is mainly degraded to 5-hydroxyindole acetic acid (5-HIAA) or melatonin and can be excreted in the urine. In the bloodstream, the vast majority of serotonin is found in platelets and can be readily detected in serum. Altered serotonin levels in serum and/or urine can indicate both physical and psychological dysfunction. Serotonin balance may be impaired in serum and/or urine in a variety of conditions. For example, decreased serotonin levels have been demonstrated in depression, anxiety, and even pain sensitivity compared to unaffected subjects. Increased serotonin levels, on the other hand, have been reported in patients with serotonin-secreting neuroendocrine tumors, also called carcinoid tumors, or hepatocellular carcinomas. Therapeutic consequences should never be based on laboratory results alone, even if these results are assessed in accordance with the quality criteria of the method. Any laboratory result is only a part of the total clinical picture of the patient. Only in cases where the laboratory results are in an acceptable agreement with the overall clinical picture of the patient, it can be used for therapeutic consequences.

Description:
Enzyme Immunoassay for the quantitative determination of serotonin in urine and serum, to evaluate serotonin homeostasis. The determination of serotonin in urine is helpful for the assessment of neurostress. The quantitative determination of serotonin follows the basic principles of the enzyme immunoassay. In the first step, serotonin is quantitatively acylated. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples compete with the solid phase bound analytes for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate resulting in a colour reaction. The reaction is monitored at a wavelength of 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations. Manual processing of the ELISA is recommended. The use of automatic laboratory equipment is the responsibility of the user. This in-vitro diagnostic is for professional use only.

Product features:
The kit contains reagents for 96 determinations;
Microtiter plate consisting of 12x8;
Microtiter plate reader at 450 nm;
Limit of detection: 5,9 ng/mL;
IVD.