Haptoglobin canine & feline ELISA

>Background:Haptoglobin, an acute phase protein, is part of immune system-mediated defence mecha- nisms found in the blood various animal species. Under normal conditions, haptoglobin is either absent from the blood or present at very low levels. However, haptoglobin can increase significantly in response to acute infection, inflammation or trauma. Several functional properties of haptoglobin have been described. The major biological func- tion of haptoglobin is to bind free hemoglobin in an equimolar ratio with very high affinity to prevent hemoglobinmediated renal parenchymal injury and loss of iron following intravascular hemolysis¹. The complex of haptoglobin with hemoglobin is metabolized in the hepatic reticulo- endothelial system. Biosynthesis of haptoglobin occurs not only in the liver, but also in adipose tissue and lung, providing antioxidant and antimicrobial activity². Haptoglobin can be used as a global parameter for inflammatory processes, since it is not diseasespecific. This is underlined by several studies examining the haptoglobin serum levels in relation to different diseases or surgical interventions. Haptoglobin can be used as one of the major indexes in addition to other acute phase proteins like C-reactive protein and cerulo- plasmin to examine the reaction in dogs3, 4, 5. In these studies, a slow increase in serum con- centration of haptoglobin is described with maximum values two to seven days after surgery. All studies strengthened the statement that haptoglobin is an alternative to serum amyloid A or fibrinogen as a diagnostic instrument for an acute phase reaction. In combination with serum amyloid A haptoglobin can be used to diagnose the inflammatory kinetic. Haptoglobin can as well be used to monitor the response of treatments.

Description:
The Haptoglobin feline & canine EIA Kit is a 96 well sandwich ELISA product. The assay uses affinity purified anti- haptoglobin antibodies immobilized at solid phase (microtiter wells). Pre-diluted samples are incubated in the microtiter wells for 60 min. The microtiter wells are sub- sequently washed, and horseradish peroxidase (HRP) conjugated antihaptoglobin antibodies are added and incubated for 30 minutes for detection. After incubation, the unbound HRP-labeled antibodies are washed away. TMB substrate is added which reacts with the HRP and resulting in a concentration-depended color level. The reaction is stopped with HCl changing the blue color to yellow, and the plate is read using a plate reader at 450 nm. Color development is proportional to the amount of haptoglobin in the sample.

Product features:
The kit contains reagents for 96 determinations;
Microtiter plate consisting of 12x8 (breakapart);
Microtiter plate reader at 450 nm;
Analytical sensitivity: 22.9 ng/mL.