Cookies help us deliver our services. By using our services, you agree to our use of cookies.
Products specifications
| Technology | qPCR / Real Time PCR/ PCR |
| Sizes | 50 preps/ kit |
Description
Total RNA isolation: for purification of high quality miRNAs, from fresh blood, cultured cells, tissue, and FFPE
PRODUCT:
Standard protocols for the isolation of total RNA or mRNA are not optimized for the purification of small RNA molecules, resulting in the loss of considerable amounts of micro RNAs (miRNAs) and other small cellular RNAs. Moreover, for accurate analysis of expression of miRNAs by qPCR or by microarray analysis requires the removal of the larger RNA molecules. The GRS microRNA Kit provides an efficient and fast method for the purification of high quality miRNAs (and other small cellular RNAs), with minimal contamination from large RNA molecules and genomic DNA, from a variety of samples, including fresh blood, cultured cells, tissue, and FFPE.
Principle: The GRS microRNA Kit uses chaotropic salts and various ethanol concentrations to allow selective binding of RNA of specific sizes to the glass fiber matrix of the spin column. Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified RNA is subsequently eluted with a specific RNAse-Free Elution Buffer. The entire procedure can be completed within 30 minutes.
Sample : up to 200μl of fresh blood, up to 1×10^6 cultured cells, up to 100mg of tissue, up to 25 mg of formalin-fixed paraffin-embedded tissue (FFPE)
Expected Yield : miRNA (and other small cellular RNAs)
Format : spin column (certified DNAse/RNAse-free)
Operation Time : 30 minutes
Elution Volume : 50μl
PRODUCT:
Standard protocols for the isolation of total RNA or mRNA are not optimized for the purification of small RNA molecules, resulting in the loss of considerable amounts of micro RNAs (miRNAs) and other small cellular RNAs. Moreover, for accurate analysis of expression of miRNAs by qPCR or by microarray analysis requires the removal of the larger RNA molecules. The GRS microRNA Kit provides an efficient and fast method for the purification of high quality miRNAs (and other small cellular RNAs), with minimal contamination from large RNA molecules and genomic DNA, from a variety of samples, including fresh blood, cultured cells, tissue, and FFPE.
Principle: The GRS microRNA Kit uses chaotropic salts and various ethanol concentrations to allow selective binding of RNA of specific sizes to the glass fiber matrix of the spin column. Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified RNA is subsequently eluted with a specific RNAse-Free Elution Buffer. The entire procedure can be completed within 30 minutes.
Sample : up to 200μl of fresh blood, up to 1×10^6 cultured cells, up to 100mg of tissue, up to 25 mg of formalin-fixed paraffin-embedded tissue (FFPE)
Expected Yield : miRNA (and other small cellular RNAs)
Format : spin column (certified DNAse/RNAse-free)
Operation Time : 30 minutes
Elution Volume : 50μl
Similar Products
GRS Pure RNA Kit
Total RNA isolation: for purification and/or concentration from samples containing partial purified RNA
0.9445924758911133
GRS Genomic DNA Kit - BroadRange
Genomic DNA purification: for whole and frozen blood, serum, plasma, buffy coat, amniotic fluid, buccal swab, hair, tissue, rodent tails, ear-punches, FFPE and insect cells
0.9323025643825531
GRS Pure DNA Kit
Genomic DNA purification: for purification and/or concentration from samples containing partial purified DNA
0.9265989810228348
0.9218832850456238
GRS Genomic DNA Kit - Tissue
Genomic DNA purification: for tissues, like tailsnips, liver, kidney, brain, adipose tissue, earpunches, insects and FFPE (available adaptation, for amniotic fluid, buccal swab, soil and stool)