Xpert Mycoplasma PCR Detection Kit

Undetected mycoplasma contamination can have significant negative impact on cell culture studies as it may affect proliferation, metabolism, gene synthesis and adhesion properties. Therefore, it is very important to detect, and possibly eliminate, any infection. This Xpert Mycoplasma PCR Detection Kit provides a fast, sensitive and reliable method for the detection of Mycoplasma and Acholeplasma species based on PCR using precisely designed specific primers. Over 200 different species of Mycoplasma and Acholeplasma can be detected, including M. orale, M. fermentans, M. hominis, M. arginini, M. hyorhinis, M. pneumoniae and A. laidlawii . The whole procedure can be carried out within 90-120 minutes, without the need of DNA isolation and with a sensitivity of as little as 10 cfu/ml.

An internal control (IC) is present in the Xpert Myco Mix, which results in a 180bp PCR product that should be present in all samples in order to minimize the risk of false negatives. Moreover, the kit contains a positive control, which should result in a product of approximately 500bp. After PCR, samples can be loaded directly onto an agarose gel without the need of adding a loading dye. Positive samples result in PCR products in the range of 300bp-600bp, depending of the species and strain.

In case samples are found to be Mycoplasma/Acholeplasma-positive, we recommend to treat the cell culture with GTC31.0001.