Xpert Fast HRM

Contains all components, except specific primers and template, for HRM analysis in qPCR.

Product description:
Main application: High Resolution Melt (HRM) analysis.
Xpert Fast HRM (2X mastermix) contains all components, except specific primers and template, for HRM analysis in qPCR.
It consists of the combination of a highly efficient hot-start enzyme with a novel low inhibitory with an optimized buffer system that allows for efficient amplification of GC-rich and AT-rich templates under both normal and fast qPCR conditions.
The intercalating dye used in this mastermix causes no inhibition of the PCR reaction thus allowing for extremely high sensitivity and specificity, as well as preventing the formation of unwanted primer-dimers and non-specific products.

Features:
Properties:
Excellent signal with low PCR inhibition
Early Ct values – Rapid extension rate
Extreme sensitivity – increased limit of detection
Allows for standard and fast cycling

Applications:
High Resolution Melt (HRM) analysis

Components:
Xpert Fast HRM is supplied in multiples of 1mL, for your convenience!
One ml of mastermix (2X), containing an optimized ratio of a fast hot-start DNA polymerase, dNTPs, MgCl2, and non-PCR inhibiting intercalating green dye, is sufficient for 100 rxns of 20μl.

Storage:
-20ºC for at least 1 year