ViroReal® Kit Deformed Wing Virus

Detection of the RNA of the polyprotein gene of deformed wing virus.

Background:Deformed wing virus is a single-stranded RNA virus of the family Iflaviridae that causes deformed wings and abdomens of adult bees and shorten their lifespan dramatically. It is found worldwide and became one of the most important threats to honeybees and other pollinators. It has often been found as a coinfection with Varroa mites. The correlation of mite burden, virus load and severity of disease suggests that Varroa acts not only as a vector but also as a viral replicator. Other ways of transmission happen via feeding, and vertically through queen’s ovaria and drone sperm.

Description:ViroReal® Kit Deformed Wing Virus is a real-time PCR for the qualitative detection of the RNA of the polyprotein gene of deformed wing virus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: RNA of the polyprotein of Deformed Wing Virus
- Transcription with thermostable MMLV Reverse Transcriptase
- Contains RNase Inhibitor to block RNA degradation
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix)