ViroReal® Kit Bovine Norovirus

Detection of RNA of the ORF1 of bovine norovirus genotypes GIII.1 and GIII.2

Background: Noroviruses are non-enveloped, single-stranded, positive-sense RNA viruses ((+)ssRNA), belonging to the family Caliciviridae. Strains are classified in five genotypes (GI – GV), infecting different species, including human, bovine, swine, mice and oysters. Bovine norovirus belong to GIII. Norovirus GIII includes two genotypes (GIII.1 and GIII.2), with genotype GIII.1 represented by jena virus and GIII.2 represented by newbury-2 virus. Infection is associated with gastrointestinal manifestations such as diarrhea, villus atrophy and crypt hyperplasia of the proximal small intestine in cattle, especially in young calves.

Description: ViroReal® Kit Bovine Norovirus uses one-step reverse transcription RT real-time PCR for the detection of ORF1 of so far known bovine norovirus genotypes GIII.1 and GIII.2. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

Product Features:
- Amplification and detection: ORF1 of Bovine Norovirus
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- Contains RNase Inhibitor to block RNA degradation
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX