REAL TIME PCR DETECTION TEST KIT beverages Brettanomyces/Dekkera

Detection Brettanomyces bruxellensis in wine and other beverages. The kit is suitable for use in real time instruments with FAM and ROX detection channels.

BACKGROUND: The yeast Brettanomyces bruxellensis (teleomorph Dekkera bruxellensis) is a spoilage yeast found in wine and various alcoholic beverages and can be found on grape berries, cellar equipment, and barrels. B. bruxellensis has been described as the principal-agent responsible for the formation of volatile phenols namely 4-ethylphenol and 4-ethylguaiacol which leads to the characteristic “Brett” taints associated with red wine, developed during wine aging and bottling. At this stage, the wines are poorly protected via the appropriate use of fungicides and due to the resistance of B. bruxellensis to sulfur dioxide control of these yeasts becomes difficult. Moreover, B. bruxellensis viability in wine seems to be enhanced by its ability to use alternative carbon sources, therefore being able to survive in the wine long after fermentation. Detection of B. bruxellensis in wine is usually performed using traditional microbiological techniques, based on culturing the organism on appropriate media and incubation for 1–2 weeks, which can have important economic consequences to wine and alcoholic beverages industry. Thus, in vitro amplification of DNA by polymerase chain reaction (PCR), in particular, using real time PCR and specific fluorescent probes, is a powerful and reliable alternative for B. bruxellensis detection, providing results in much shorter times and allowing more efficient control procedures.

PRODUCT DESCRIPTION: REAL TIME DETECTION KIT Brettanomyces/Dekkera bruxellensis uses real-time PCR for the detection of Brettanomyces bruxellensis (teleomorph Dekkera bruxellensis) in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target microorganism. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the target microrganism as well as a positive control and an internal control (IC).

PRODUCT FEATURES:
-Amplification and detection of Brettanomyces bruxellensis (teleomorph Dekkera bruxellensis)
-Real-time PCR with rapid hot-start Taq DNA polymerase
-Internal Control to exclude false-negative results
-PCR- platforms: runs on all established standard real-time PCR- platforms
-Harmonized thermal profiles to run with other BIOPREMIER kits simultaneously
-Channel Target: FAM, Channel IC: ROX
-Available formats: Kits with 100 or 10 reactions