Real Time PCR Detection Kit Panel Zoonotic Bacterial Diseases

The Real Time PCR detection Kit Panel Zoonotic Bacterial Diseases (Brucella spp., Bacillus anthracis, Francisella tularensis and Yersinia pestis) comprises a series of specific targeted reagents designed for Brucella spp., Bacillus anthracis, Francisella tularensis and Yersinia pestis detection by using qPCR

GENERAL INFORMATION:Zoonotic diseases from microorganisms pose significant public health risks. They can easily transmit from animals to humans, leading to severe infections. Brucella is a genus of small, non-motile, Gram-negative coccobacilli that function as intracellular parasites. It causes brucellosis, transmitted through contaminated food, animal contact, or aerosols. Symptoms include fever, joint pain, and fatigue. Bacillus anthracis is a Gram-positive, endospore-forming bacterium and the causative agent of anthrax, affecting livestock and sometimes humans. Its endospores are resilient, surviving harsh conditions for decades. Anthrax symptoms vary by infection route but include skin ulcers, respiratory distress and gastrointestinal issues. Francisella tularensis is a Gram-negative bacterium causing tularemia, or “rabbit fever”. It spreads via insect bites, inhalation, or contact with infected animals. Symptoms include fever, swollen lymph nodes, and respiratory problems. Due to its high virulence and ease of spread, it’s a potential bioterrorism agent. Yersinia pestis is a Gram-negative, non-motile bacterium responsible for plague, a highly infectious zoonotic disease. It primarily spreads through flea bites or direct contact with infected animals. Plague presents in three forms: bubonic (swollen lymph nodes), septicemic (blood infection), and pneumonic (lung infection). Due to its rapid transmission and high fatality rate if untreated, Y. pestis has caused major pandemics.

KIT CHARACTERISTICS:
- Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
- Resuspension buffer
- DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control