Real Time PCR Detection Kit Newcastle disease virus classes 1&2 (NDV-1&2)

The Real Time PCR detection Kit Newcastle disease virus classes 1&2 (NDV-1&2) comprises a series of specific targeted reagents designed for Newcastle disease virus class 1 and class 2 detection by using qPCR.

GENERAL INFORMATION:Newcastle disease virus is a single-stranded, negative-sense RNA virus belonging to the Avulavirus genus within the Paramyxoviridae family. It is responsible for a contagious disease affecting many domestic and wild avian species and is also transmissible to humans. Newcastle disease is characterized by respiratory, nervous, and digestive system symptoms, which can range from mild to severe, depending on the virulence of the strain and the species of bird affected. Transmission occurs through direct contact with infected birds, their droppings, or contaminated equipment and environments. Its effects are most notable in domestic poultry due to their high susceptibility and the potential for severe impacts on the poultry industry. The virus is endemic to many countries. Newcastle disease was first identified in Newcastle upon Tyne, United Kingdom, in 1926, and later by Burnet in 1943 in Australia in connection with laboratory infection. Based on phylogenetic analyses, Newcastle disease viruses are classified into two distinct classes, class 1 and class 2. Class 1 strains have been isolated mainly from wild birds and live bird markets and are generally avirulent. Class 2 strains include a range of avirulent to virulent isolates from several species of wild birds and domestic poultry species. The majority of the class 2 strains are virulent and are responsible for most of the outbreaks worldwide.

KIT CHARACTERISTICS:
- Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
- Resuspension buffer
- DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control