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Products specifications
| Technology | qPCR / Real Time PCR/ PCR |
| Sizes | 4 targets: 4 x 100 reactions - FAM 4 targets + IC: 4 x 100 reactions - FAM, HEX Monodose 4 targets: 4 x 96 qPCR tubes - FAM Monodose 4 targets + IC: 4 x 96 qPCR tubes - FAM, HEX |
Description
Background:Pathogens responsible of brown rot Monilinia fructicola, Monilinia laxa, Monilinia fructigena and Monilinia polystroma detection by using qPCR.
Brown rot is one of the most destructive diseases of stone fruits, such as peach, nectarine, apricot, cherry, and plum. Three species of Monilinia and the recently described anamorph, Monilia polystroma (formerly lumped into M. fructigena), cause brown rot in temperate regions on members of the Rosaceae. M. fructicola colonizes blossoms, twigs and fruit, primarily on Prunus spp., but also on pome fruit or other Rosaceae. M. laxa can colonize blossoms, twigs and fruit, but is generally considered more of a problem on blossoms and twigs. M. fructigena is primarily a problem on the fruit of apple, pear, and other pome fruits. M. polystroma is reported to cause rot on both pome and stone fruits. The brown rot fungus most commonly overwinters on fruit mummies on the tree and on the ground, in infested crop debris, and in cankers. The spread of the fungal spores to new infection sites occurs by wind and rain-splash. Insects, such as honeybees or beetles, may also transport the fungal inoculum to new infection sites. Monilinia spp. occur throughout the world.
Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control
Brown rot is one of the most destructive diseases of stone fruits, such as peach, nectarine, apricot, cherry, and plum. Three species of Monilinia and the recently described anamorph, Monilia polystroma (formerly lumped into M. fructigena), cause brown rot in temperate regions on members of the Rosaceae. M. fructicola colonizes blossoms, twigs and fruit, primarily on Prunus spp., but also on pome fruit or other Rosaceae. M. laxa can colonize blossoms, twigs and fruit, but is generally considered more of a problem on blossoms and twigs. M. fructigena is primarily a problem on the fruit of apple, pear, and other pome fruits. M. polystroma is reported to cause rot on both pome and stone fruits. The brown rot fungus most commonly overwinters on fruit mummies on the tree and on the ground, in infested crop debris, and in cankers. The spread of the fungal spores to new infection sites occurs by wind and rain-splash. Insects, such as honeybees or beetles, may also transport the fungal inoculum to new infection sites. Monilinia spp. occur throughout the world.
Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control
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