Real Time PCR Detection Kit Botrytis spp.

The Real Time PCR Detection Kit Borrelia spp. comprises a series of specific targeted reagents designed for Botrytis spp. detection by using qPCR.

GENERAL INFORMATION:Botrytis species are filamentous fungi of the family Sclerotiniaceae. Commonly known as grey mold, Botrytis comprises over 30 species, most of which are necrotrophic pathogens that kill host cells and subsequently decompose plant tissue. The most notable species, B. cinerea, causes grey mold disease in a wide range of dicotyledonous plants, including grapes, strawberries, tomatoes, and ornamentals. In viticulture, B. cinerea is responsible for bunch rot, although under specific environmental conditions, it can cause noble rot, a desirable infection used in the production of sweet botrytised wines.

Other species of economic importance cause diseases such as neck rot and leaf blight in onions. Infected plants typically exhibit water-soaked lesions, grey spore masses, and rapid tissue decay, leading to significant losses in yield and quality. Transmission occurs mainly through airborne conidia or direct contact with contaminated plant material. Botrytis species are globally distributed and thrive in humid, temperate climates. Target sequences for Botrytis allii were not available at the time of PCR design. However, given the high degree of sequence conservation at this locus among Botrytis species, full inclusivity for B. allii is expected.

KIT CHARACTERISTICS:
- Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
- Resuspension buffer
- DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control