Real Time PCR Detection Kit Actinobacillus pleuropneumoniae omIA III + IV

The Real Time PCR detection Kit Actinobacillus pleuropneumoniae omIA III + IV comprises a series of specific targeted reagents designed for Actinobacillus pleuropneumoniae omlA III + IV detection by using qPCR.

GENERAL INFORMATION:Actinobacillus pleuropneumoniae (previously Haemophilus pleuropneumoniae) is a non-motile, Gram-negative, encapsulated coccobacillus from the family Pasteurellaceae. It causes porcine pleuropneumonia, a highly contagious respiratory disease in young pigs, particularly under 6 months old. Symptoms include respiratory distress, bloodstained discharge, fever, cyanosis, anorexia, and spontaneous abortion in sows. Mortality rates can reach 20-80% in fattening pigs, with high morbidity. Survivors often become carriers, further spreading the infection. There are two biotypes and at least 15 serotypes, all of which can cause disease. The variation in virulence between serotypes is attributed to the differential expression of virulence factors, including the omlA gene, which encodes a key outer membrane lipoprotein crucial for pathogenicity. The omlA III variant is associated with highly virulent strains. The omlA IV variant in A. pleuropneumoniae is associated with strains of intermediate or lower virulence.

KIT CHARACTERISTICS:
- Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
- Resuspension buffer
- DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control