Real Time PCR Detection Kit Actinobacillus pleuropneumoniae Apx I toxin

The Real Time PCR detection Kit Actinobacillus pleuropneumoniae Apx I toxin comprises a series of specific targeted reagents designed for Actinobacillus pleuropneumoniae Apx I toxin detection by using qPCR.

GENERAL INFORMATION:Actinobacillus pleuropneumoniae (previously Haemophilus pleuropneumoniae), is a non-motile, Gram-negative, encapsulated coccobacillus bacterium found in the Pasteurellaceae family. It is a respiratory pathogen found in pigs was found to be the causative agent for up to 20% of all bacterial pneumonia cases in swine. The main disease associated with this bacterium is porcine pleuropneumonia, a highly contagious respiratory disease, affecting primarily young pigs (usually less than 6 months). The symptoms include respiratory distress, bloodstained discharge (usually frothy) from the mouth, fever, anorexia, mild diarrhoea, cyanosis, lethargy, and spontaneous abortion in sows. Mortality rates can reach 20-80 % in fattening pigs, with similarly high morbidity. Pigs that do survive the disease remain as carriers and spread the bacterium to other swine. A. pleuropneumoniae has a profound economic impact on pork production and pig farmers. There are two biotypes and at least 15 serotypes. Key virulence factors include Apx toxins, which are divided into four types. Apx I toxin is an exotoxin that is strongly haemolytic and cytotoxic, causing cell lysis, which results in lung damage and hemorrhaging in infected pigs.

KIT CHARACTERISTICS:
- Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
- Resuspension buffer
- DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control