ParoReal® Kit Ascaris

Detection of DNA of the ITS1 region of Ascaris spp.

Background:Ascaris, also known as roundworm, is a parasitic nematode that causes ascariasis in the small intestine of pigs. Ascaris can infect other animals, such as chickens and cattle and cross-infections between humans and pigs are possible. Roundworms in pigs and humans are considered as two species (A. suum and A. lumbricoides). Female worms can lay up to 200,000 eggs per day. The eggs are covered with a sticky protective coat and can remain infective for years in the soil. In the mild cases, which are most common, the only evidence of the worms is at the time of slaughter where milk spot-like lesions can be seen on the liver ("milk spot"). In heavy cases, which are extremely rare, the worms can block the intestine leading to vomiting, weight loss and death.

Description:ParoReal® Kit Ascaris is a real-time PCR test for the qualitative detection of DNA of the ITS1 region of Ascaris spp. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: DNA of the ITS1 region of Ascaris
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously