ParoReal® Kit Malpighamoeba mellificae

Detection of the rRNA gene of Malpighamoeba mellificae.

Background:Malpighamoebia mellificae​ is related to the family of ​Amoebidae​ and effects, as a single-celled parasite, the excretory organs (malphigian tubules) of adult bees, but only of the western honeybee ​Apis mellifera​​. The intake cysts develop into trophozoites and damage the epithelia of the tubule. That causes disbalance between excretion and haemolymph exchange and leads to amoebiasis, that increases sudden mortality. Since the phase of excreting cysts is intermittent, or could even not happen during a longer period, there is a lack of microscopy detection. Often co-infections with Nosema are observed.

Description:ParoReal® Kit Malpighamoeba mellificae is a real-time PCR for the qualitative detection of the rRNA gene of Malpighamoeba mellificae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: rRNA gene of Malpighamoeba mellificae
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously