MFIA Test Actinobacillus pleuropneumoniae (APP) (1-9-11 2 3-6-8-15 4-7 & 5) Multiplex Swinecheck

Mutiplex immunoassay intended for determining the presence of antibodies to Actinobacillus pleuropneumoniae (APP) serogroups 1-9-11, 2, 3-6-8-15, 4-7, and 5 in swine serum

DESCRIPTION:
Swinecheck MP® APP assay is a mutiplex immunoassay intended for determining the presence of antibodies to Actinobacillus pleuropneumoniae (APP) serogroups 1- 9-11, 2, 3-6-8-15, 4-7, 5, 10 and 12 in swine serum.

SUMMARY AND EXPLANATION:
Swinecheck MP® APP assay is a microsphere-based multiplex fluorescent immunoassay (MFIA)-type antibody detection test. A total of 7 distinct microsphere sets (coated with either APP 1-9-11, APP 2, APP 3-6-8-15, APP 4-7, APP 5, APP 10 and APP 12 antigens) are incubated in diluted swine serum and then washed. Successive incubations with biotinylated anti-swine IgG (Detection Antibody) and streptavidin R-phycoerythrin (SA-PE) reporter follow, each succeeded by a wash step to remove unbound reagent. Users place plates in the plate analyzer, which captures the fluorescence intensity of the microspheres and SA-PE reporter. An S/P ratio of the SA-PE on the antigen-coated microspheres above the provided cut-offs indicates antibody is present in the sample.

CHARACTERISTICS:
- Multiplexed fluorometric immunoassay (MFIA)
- 5 plates
- Results available within 2.5 hours
- Tests per kit: up to 445 samples