Picture of HEPES Buffer Solution (1 M)

HEPES Buffer Solution (1 M)

Brand: Research Solutions
SKU: GTC14.0100
Solutions commonly used in cell culture applications
+ -
Products specifications
TechnologyOther
Sizes100mL
Description
Solutions commonly used in cell culture applications

PRODUCT:
HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is widely used as a buffering agent in cell culture media. The zwitterion possesses a pKa(1) of 3 and a pKa(2) of 7.5 with useful pH ranges of 2.5-3.5 and 6.7-8.6. In comparison with bicarbonate buffer systems, HEPES is better in maintaining physiological pH despite changes in CO2 concentrations resulting from cellular activity.
HEPES is suitable for many cell culture systems as it is membrane impermeable and has limited effect on biochemical reactions.

Quantity: 100ml

Appearance: Clear colourless solution.
Similar Products
Picture of Acrylamide/Bisacrylamide Solution

Acrylamide/Bisacrylamide Solution

High purity solutions and reagents for the preparation of polyacrylamide gels for protein electrophoresis
Picture of Xpert Enhancer Blocking Solution

Xpert Enhancer Blocking Solution

Xpert Enhancer Blocking Solution is a 2-in-1 Buffer for Western Blotting; not only can membrane blocking and antibody hybridization be carried out in a single-step, saving considerable amounts of time, the buffer also enhances the signal developed with either HRP (horseradish peroxidase) or AP (alkaline phosphatase) substrates
Picture of GRS Stripping Solution

GRS Stripping Solution

High purity solutions for staining and for antibody stripping applications
Picture of Ponceau S Solution

Ponceau S Solution

High purity solutions for staining and for antibody stripping applications
Picture of G-418 Sulphate Solution (50mg/ml)

G-418 Sulphate Solution (50mg/ml)

Solution used for the selection and maintenance of eukaryotic cells, stably transfected with neomycin resistance genes.