Cookies help us deliver our services. By using our services, you agree to our use of cookies.
Products specifications
| Technology | ELISA |
| Sizes | 96 tests |
Description
Description:
The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzymelinked Immunosorbent Assay) technique. Microtiterplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour.
Product features:
The kit contains reagents for 96 determinations;
Microtiter plate reader at 450/620 nm;
Cut-off: 10 U
The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzymelinked Immunosorbent Assay) technique. Microtiterplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour.
Product features:
The kit contains reagents for 96 determinations;
Microtiter plate reader at 450/620 nm;
Cut-off: 10 U
Similar Products
Coxiella Phase 1 vet ELISA
Enzyme immunoassay for the qualitative determination of antibodies against Coxiella Phase 1 in veterinary serum, pooled serum samples and milk samples
0.9999999985191501
ELISA TEST Leptospira pomona and hardjo Bovichek
Leptospira pomona and hardjo Antibody Test Kit, ELISA
0.9433657377958298
Real Time PCR Detection Kit Mycoplasma haemominutum (Candidatus)
The Real Time PCR detection Kit Mycoplasma haemominutum (Candidatus) comprises a series of specific targeted reagents designed for Mycoplasma haemominutum (Candidatus) detection by using qPCR.
0.9425728879868984
Real Time PCR Detection Kit Mycoplasma haematoparvum (Candidatus)
The Real Time PCR detection Kit Mycoplasma haematoparvum (Candidatus) comprises a series of specific targeted reagents designed for Mycoplasma haemotoparvum (Candidatus) detection by using qPCR.
0.9425728879868984
Real Time PCR Detection Kit Mycoplasma turicensis (Candidatus)
The Real Time PCR detection Kit Mycoplasma turicensis (Candidatus) comprises a series of specific targeted reagents designed for Mycoplasma turicensis (Candidatus) detection by using qPCR.