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BactoReal® Kit Brachyspira hyo/pilo
BactoReal® Kit Brachyspira hyo/pilo
Brand:
Ingenetix
Combined detection of the nox gene of Brachyspira hyodysenteriae and Brachyspira pilosicoli. No differentiation between species
SKU:
DVEB01811
Choose the product variant:
*
DVEB01853: 50 reactions - FAM, Cy5
DVEB01851: 50 reactions - FAM, VIC/HEX
DVEB01813: 100 reactions - FAM, Cy5
DVEB01811: 100 reactions - FAM, VIC/HEX
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Products specifications
Technology
qPCR / Real Time PCR/ PCR
Sizes
DVEB01811: 100 reactions - FAM, VIC/HEX
DVEB01813: 100 reactions - FAM, Cy5
DVEB01851: 50 reactions - FAM, VIC/HEX
DVEB01853: 50 reactions - FAM, Cy5
Combined detection of the nox gene of Brachyspira hyodysenteriae and Brachyspira pilosicoli. No differentiation between species
Background:
Brachyspira are anaerobic intestinal spirochaetes. Brachyspira hyodysenteriae (previously called Serpulina hyodysenteriae or Treponema hyodysenteriae) is the causative agent of swine dysentery. Swine dysentery is a disease characterized by mucohaemorrhagic diarrhoea with lesions confined to the large intestine of pigs. Its incubation time is 10-14 days. The faecal excretion of B. hyodysenteriae starts 2 days after infection. Brachyspira pilosicoli (previously called Serpulina pilosicoli) colonizes the large intestine of various species, including humans. It is the etiologic agent of human and animal intestinal spirochetosis and rarely implicated as a cause of bacteremia. For example, it is the causative agent of porcine colonic spirochaetosis (PCS). Brachyspira are anaerobic bacteria but are aerotolerant due, at least in part, to high NADH oxidase activity.
Description:
BactoReal® Kit Brachyspira hyo/pilo uses real-time PCR for the detection of the NADH oxidase (nox) gene of B. hyodysenteriae and B. pilosicoli. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG) to minimize PCR cross-contamination
Product Features:
- Amplification and detection: NADH nox gene of B. hyodysenteriae and B. pilosicoli
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX
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