ZnT8 Ab ELISA

Enzyme immunoassay for the quantitative determination of ZnT8 Ab in human serum.

INTENDED USE:The RSR ZnT8 autoantibody (ZnT8 Ab) ELISA kit is intended for use by professional persons only, for the quantitative determination of ZnT8 Ab in human serum.

GENERAL INFORMATION:Autoantibodies to pancreatic beta cell antigens are important serological markers of type 1 diabetes mellitus (type 1 DM). The antigens recognised by these antibodies include insulin, glutamic acid decarboxylase (GAD65 kDa isoform), the islet cell antigen IA-2 or ICA-512 and zinc transporter 8 (ZnT8). ZnT8 Ab are directed principally to the C terminal domain of ZnT8 (residues 268 – 369). Human population gene polymorphism at the codon for the 325th amino acid results in the expression of three protein variants: Arginine (R) 325, Tryptophan (W) 325 and very rarely Glutamine (Q) 325. ZnT8 Ab may be specific to the R 325 or W 325 variant, or may be residue 325 non-specific. Sera that react with the Q allele only are extremely rare. RSR’s ZnT8 Ab ELISA is capable of detecting, and quantifying, autoantibodies specific to R 325 or to W 325, or to residue 325 non-specific variants.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 16-20 h, 1 h (2-8°C), 20 min (RT/shaker), 20 min (RT/dark)
- Standard Range: 10 - 2,000 U/ml
- Sensitivity analytical: 1.2 U/ml
- Final Sample Volume: 25 µl
- Sample Type: serum
- Isotope / Substrate: TMB 450 nm
- Internal Controls: 3
- Regulatory Status: CE