VOLTage Fast Electrophoresis Buffer
VOLTage™ Fast Electrophoresis Buffer is for Agarose Electrophoresis of DNA fragments of 100bp to 1000bp. It is much faster than TBE with higher resolution and better separation.
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Technology Other Sizes GB02.0120: 1L 20X GB02.0520: 5L 20X
VOLTage™ Fast Electrophoresis Buffer is for Agarose Electrophoresis of DNA fragments of 100bp to 1000bp. It is much faster than TBE with higher resolution and better separation.GENERAL INFORMATION: VOLTage Fast Electrophoresis Buffer is ideal for agarose gel electrophoresis of small DNA fragments (up to 1000bp). Unlike gels prepared with TAE or TBE, which tolerate only about 110V (mini-gel), this low-conductance electrophoresis buffer allows for the application of high voltage (200V–300V for a mini-gel) without generating a lot of heat, and without melting your gel. This allows for much faster runs. Not only does this save time, but faster runs result in less fuzzy bands, and therefore, using VOLTage Fast Electrophoresis Buffer results in very sharp bands. Moreover, in comparison with TBE and TAE, using VOLTage Fast Electrophoresis Buffer results in larger differences in relative mobility (Rf), especially between smaller DNA fragments, allowing for a better separation of DNA bands of similar size. On the other hand, if you already get the resolution you need, with VOLTage Fast Electrophoresis Buffer you can achieve the same result using less agarose, thus not only saving you time but money as well. Finally, gels prepared with VOLTage Fast Electrophoresis Buffer are clearer than gels prepared with TAE, increasing detection sensitivity, and also stronger, making them easier to handle.KIT CHARACTERISTICS: - Time Saving: Unlike TAE and TBE gels, which tolerate only about 110V, this low-conductance electrophoretic buffer allows for the application of high voltage 200-300V without generating a lot of heat, so your gel won´t melt and thus FASTER RUNS - High Resolution: DNA agarose gel electrophoresis using VOLTage™ Fast Electrophoresis Bufferresults in very sharp bands. Faster Runs result in less fuzzy bands. - Better Separation: Using VOLTage™ Fast Electrophoresis Buffer results in larger differences in relative mobility (Rf), especially between smaller DNA fragments. - Stronger and Clearer: Gels made with VOLTage™ Fast Electrophoresis Buffer are also CLEARER, increasing detection sensitivity; and STRONGER, making them excelent for blotting.