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Products specifications
| Technology | qPCR / Real Time PCR/ PCR |
| Sizes | 48 rxn |
Description
The VIRSeek Solution offers an all-inclusive, ISO 15216-compliant workflow from RNA extraction to the specific detection of norovirus genogroup I, norovirus genogroup II and hepatitis A virus. In addition, the process control murine norovirus (MNV) ensures monitoring of the RNA isolation procedure. Each of the VIRSeek Solution kits can be used individually or as part of the comprehensive workflow, validated for the Agilent AriaMx™ and Bio-Rad CFX96™ (Standard and Deep Well) platforms.
The VIRSeek Food Hepatitis A Virus real-time RT-PCR kit provides all reagents for the rapid detection of the hepatitis A virus in a number of food products including soft fruits, leaf, stem and bulb vegetables, bottled water, bivalve molluscan shellfish as well as on food surfaces. A viral concentration prior to RNA extraction is required for these sample types. The kit is intended to be used by analytical laboratories for quality control/quality assurance testing, food and agricultural testing, and research purposes only (e.g. virological monitoring of production processes).
After concentration of viral particles from food matrices and subsequent extraction of viral RNA, the VIRSeek Food Hepatitis A Virus Real-Time RT-PCR kit can be used for detection of the target virus. The first step of a real-time RT-PCR reaction is reverse transcription (RT) of viral RNA into cDNA, which is then amplified by real-time PCR. For RNA extraction, we recommend the VIRSeek RNAExtractor Food kit.
In accordance with ISO 15216-1/2, the processes and horizontal methods for the detection of hepatitis A virus and norovirus by real-time RT-PCR in food samples require the use of a process control virus to verify the correct virus concentration process from samples. Process control viruses must share similar characteristics with the target virus of interest. For this reason, the VIRSeek Food Murine Norovirus (MNV) Process Control kit is recommended for use in combination with all food-borne virus detection kits.
Related Kits:
- VIRSeek Food Hepatitis A Virus, (5728200101) — Real-time RT-PCR kit with 48 reactions for rapid detection of hepatitis A virus (HAV) in food samples.
- VIRSeek Food Hepatitis E Virus, (5728200501) — Real-time RT-PCR kit with 48 reactions for rapid detection of hepatitis E virus (HEV) in food samples.
- VIRSeek Food Norovirus Genogroup I, (5728200201) — Real-time RT-PCR kit with 48 reactions for rapid detection of norovirus genogroup I (NoVGI) in food samples.
- VIRSeek Food Norovirus Genogroup II, (5728200301) — Real-time RT-PCR kit with 48 reactions for rapid detection of norovirus genogroup II (NoVGII) in food samples.
- VIRSeek Murine Norovirus (MNV) Process Control, (5728200401) — Murine norovirus spiking material (0.5 mL) and real-time RT-PCR kit with 48 reactions for rapid detection of murine norovirus (MNV) process control virus in food samples.
- VIRSeek RNAExtractor Food, (5524401501) — Kit for extraction of viral RNA via silica-coated magnetic beads from various food products (including soft fruits, leafy, stem and bulb vegetables, bottled water and bivalve molluscan shellfish). 48 x 500 μL or 96 x 250 μL sample input volume. All reagents provided.
The VIRSeek Food Hepatitis A Virus real-time RT-PCR kit provides all reagents for the rapid detection of the hepatitis A virus in a number of food products including soft fruits, leaf, stem and bulb vegetables, bottled water, bivalve molluscan shellfish as well as on food surfaces. A viral concentration prior to RNA extraction is required for these sample types. The kit is intended to be used by analytical laboratories for quality control/quality assurance testing, food and agricultural testing, and research purposes only (e.g. virological monitoring of production processes).
After concentration of viral particles from food matrices and subsequent extraction of viral RNA, the VIRSeek Food Hepatitis A Virus Real-Time RT-PCR kit can be used for detection of the target virus. The first step of a real-time RT-PCR reaction is reverse transcription (RT) of viral RNA into cDNA, which is then amplified by real-time PCR. For RNA extraction, we recommend the VIRSeek RNAExtractor Food kit.
In accordance with ISO 15216-1/2, the processes and horizontal methods for the detection of hepatitis A virus and norovirus by real-time RT-PCR in food samples require the use of a process control virus to verify the correct virus concentration process from samples. Process control viruses must share similar characteristics with the target virus of interest. For this reason, the VIRSeek Food Murine Norovirus (MNV) Process Control kit is recommended for use in combination with all food-borne virus detection kits.
Related Kits:
- VIRSeek Food Hepatitis A Virus, (5728200101) — Real-time RT-PCR kit with 48 reactions for rapid detection of hepatitis A virus (HAV) in food samples.
- VIRSeek Food Hepatitis E Virus, (5728200501) — Real-time RT-PCR kit with 48 reactions for rapid detection of hepatitis E virus (HEV) in food samples.
- VIRSeek Food Norovirus Genogroup I, (5728200201) — Real-time RT-PCR kit with 48 reactions for rapid detection of norovirus genogroup I (NoVGI) in food samples.
- VIRSeek Food Norovirus Genogroup II, (5728200301) — Real-time RT-PCR kit with 48 reactions for rapid detection of norovirus genogroup II (NoVGII) in food samples.
- VIRSeek Murine Norovirus (MNV) Process Control, (5728200401) — Murine norovirus spiking material (0.5 mL) and real-time RT-PCR kit with 48 reactions for rapid detection of murine norovirus (MNV) process control virus in food samples.
- VIRSeek RNAExtractor Food, (5524401501) — Kit for extraction of viral RNA via silica-coated magnetic beads from various food products (including soft fruits, leafy, stem and bulb vegetables, bottled water and bivalve molluscan shellfish). 48 x 500 μL or 96 x 250 μL sample input volume. All reagents provided.