ViroReal® Kit Swine Influenza A (SIV)

Detection of RNA of the matrix protein gene of influenza A virus

Background: Swine Influenza is an acute, febrile respiratory disease of swine with high morbidity and low mortality. In the industry it is commonly known as Swine Flu, Hog Flu, and Pig Flu. It is caused by the influenza virus, mainly influenza type A, an enveloped virus with single-stranded, segmented RNA with negative polarity as genome. Influenza A virus infects a wide variety of warm-blooded animals including pigs, birds, horses as well as humans.

Description: ViroReal® Kit SIV uses one-step reverse transcription RT real-time PCR for the detection of the matrix protein gene of the influenza A virus of warm-blooded animals including pigs, birds, horses as well as humans. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

Product Features:
- Amplification and detection: matrix protein gene of Swine Influenza A
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- Contains RNase Inhibitor to block RNA degradation
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX