VEGF human ELISA

Enzyme immunoassay for the quantitative determination of Human VEGF in EDTA plasma or cell culture supernatant.

INTENDED USE:This kit is to be used for the in-vitro quantitative determination of Human VEGF in EDTA plasma or cell culture media. The assay will recognize both natural and recombinant Human VEGF. For research use only, not for use in diagnostic procedures.

GENERAL INFORMATION: Vascular Endothelial Cell Growth Factor (VEGF) is a homodimeric protein initially purified from media conditioned by normal bovine pituitary folliculo-stellate cells and secreted by a variety of vascularized tissues. It was subsequently found to be identical to a vascular permeability factor (VPF), which was previously identified in media conditioned by tumor cell lines based upon its ability to increase the permeability of capillary blood vessels. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis and capillary permeability. Human VEGF is a 38.2kDa homodimeric protein consisting of two 165 amino acid polypeptide chains. VEGF is expressed in many human tumor cells, including human adenocarcinoma, human pancreatic carcinoma, human hepatocellular carcinoma, renal cell carcinoma, fibrosarcoma, HL60 promyelocytic leukemia, GS-9L glioma and U937 lymphoma cells. In normal tissues, VEGF expression has been observed in activated macrophages, keratinocytes, hepatocytes, smooth muscle cells Leydig cells, embryonic fibroblasts and bronchial and choroids plexus epithelium, renal glomerular visceral epithelium and mesangial cells. The IBL’s Human VEGF ELISA kit is a complete kit for the quantitative determination of human VEGF in EDTA plasma and supernatant of cell culture media.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h (37°C), 30 (4°C), 30 min (RT)
- Standard Range: 15.63 - 1,000 pg/ml
- Sensitivity analytical: 1 pg/ml
- Final Sample Volume: 100 µl
- Sample Type: plasma (EDTA), cell culture supernatant
- Isotope / Substrate: TMB 450 nm
- Laboratory Status: RUO