Total Prostate Specific Antigen (PSA) ELISA

Enzyme immunoassay for the quantitative measurement of Total Prostate Specific Antigen (PSA) in human serum.

INTENDED USE:For the quantitative measurement of Total Prostate Specific Antigen (PSA) in human serum by an ELISA (Enzyme-Linked Immunosorbent Assay). For Research Use Only. Not for use in diagnostic procedures.

GENERAL INFORMATION:The Total PSA ELISA is a two-step capture or ‘sandwich’ type immunoassay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for Total PSA is immobilized onto the microplate and another monoclonal antibody specific for a different epitope of PSA is conjugated to horse radish peroxidase (HRP conjugate). In the first incubation step, PSA present in the specimen samples, calibrators and controls is bound by the antibody immobilized onto the microplate. Excess and unbound materials are removed by a washing step. In the second incubation step, HRP conjugate antibody (HRP conjugate) is added, which binds specifically to any immobilized PSA, thus forming a sandwich complex. Unbound HRP conjugate is removed by a washing step. Next, the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue coloured product that is directly proportional to the amount of PSA present. The enzymatic reaction is terminated by the addition of the stopping solution, converting the colour from blue to yellow. The absorbance is measured on a microplate reader at 450 nm. A set of calibrators is used to plot a calibrator curve from which the amount of Total PSA in specimen samples and controls can be directly read.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h, 30 min, 10-15 min (RT/shaker)
- Standard Range: 0.5 - 100 ng/ml
- Final Sample Volume: 25 µl
- Sample Type: serum
- Isotope / Substrate: TMB 450 nm
- Internal Controls: 2
- Regulatory Status: RUO