Thyroxine (T4) ELISA

Enzyme immunoassay for the quantitative determination of Thyroxine (T4) in human serum.

INTENDED USE: For the direct quantitative determination of Thyroxine by an enzyme immunoassay in human serum

GENERAL INFORMATION: The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of T4 in the sample. A set of standards is used to plot a standard curve from which the amount of T4 in patient samples and controls can be directly read.

Clinical Trends:
- The level of T4 in hypothyroid patients is decreased.
- The level of T4 in hyperthyroid patients is increased.
- In euthyroid individuals the level of T4 is within the normal range.
Thyroid binding globulin (TBG) levels have reportedly been elevated by the following conditions: increased estrogens from oral contraceptives, androgens, glucocorticords and pregnancy. Consequently, borderline T4 values should be viewed with caution when any of the above conditions are present.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 30 min, 15-20 min (RT/shaker)
- Standard Range: 1 - 32 µg%
- Sensitivity analytical: 0.6 µg%
- Final Sample Volume: 20 µl
- Sample Type: serum
- Isotope / Substrate: TMB 450 nm
- Internal Controls: 2
- Regulatory Status: CE