Syndecan-4 ELISA

Enzyme immunoassay for the quantitative determination Human Syndecan-4 in serum, EDTA-plasma and cell culture supernatant.

INTENDED USE:This assay kit is capable for the quantitative determination Human Syndecan-4 in serum, EDTA-plasma and cell culture supernatant.

GENERAL INFORMATION:The syndecan family contains four members (syndecan-1 / syndecan, syndecan-2 / fibroglycan, syndecan-3 / N-syndecan, syndecan-4 / ryudocan (amphyglycan)), which are transmembrane heparan sulfate proteoglycans (HSPGs). These HSPGs exhibit cell type-specific distribution with vascular endothelial cells expressing syndecan-1, -2, and -4, and predominant targeting to basolateral surfaces. The syndecan family members are type I integral membrane proteins with homologous transmembrane and cytoplasmic domains. The combined transmembrane / cytoplasmic domains contain four well-conserved tyrosine residues, which might serve important roles for biological function. The cytoplasmic tail of syndecan-4 interacts with focal adhesion molecules. Thus, syndecan-4 is known to have various biological functions such as anticoagulation, cell adhesion and cell multiplication.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h (37°C), 30 (4°C), 30 min (RT)
- Standard Range: 20 - 1,280 pg/ml
- Sensitivity analytical: 3.94 pg/ml
- Final Sample Volume: 100 µl
- Sample Type: serum, plasma (EDTA), cell culture supernatant
- Isotope / Substrate: TMB 450 nm
- Regulatory Status: RUO