secreted alpha-Klotho human ELISA

Enzyme immunoassay for the quantitative determination of secreted alpha-Klotho in human serum, EDTA-plasma and cerebrospinal fluids.

INTENDED USE:This product is for research use only and is not intended for diagnostic use.

GENERAL INFORMATION:This kit is a solid phase sandwich ELISA (Enzyme-linked Immunosorbent Assay). As a primary antibody is coated on a plate, samples and standard are added into the wells for 1st reaction. After the reaction, HRP-conjugated secondary antibody is added into the wells for 2nd reaction. After washing away unbound the secondary antibody, Tetra Methyl Benzidine (TMB) is added to the wells and color develops.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
-Incubation Time / Conditions: Over night (2-8°C), 1 h (2-8°C), 30 min (RT)
- Standard Range: 70.31 - 4,500 pg/ml
- Sensitivity analytical: 9.87 pg/ml
- Final Sample Volume: 100 µl
- Sample Type: serum, plasma (EDTA), cerebrospinal fluids
- Sample Pretreatment: Dilution 1:2 to 1:4
- Isotope / Substrate: TMB 450 nm
- Regulatory Status: RUO