sAPP alpha (highly sensitive) human ELISA

Enzyme immunoassay for the quantitative determination human sAPPa in serum, EDTA plasma, cerebrospinal fluids and cell culture supernatant.

INTENDED USE:This assay kit is capable for the quantitative determination human sAPPα in serum, EDTA plasma, cerebrospinal fluids and cell culture supernatant.

GENERAL INFORMATION:Alzheimer's disease (AD) was first reported by A. Alzheimer, a German neuropathologist in 1907 and is considered as a major factor of dementia. It is known that Amyloid β (Aβ; which is major constituent of senile plaque) is cleaved from Amyloid Precursor Protein (APP; which exists in three main isoforms, APP695, APP751, and APP770) by β-secretase and subsequent γ-secretase. The production of soluble APPβ (sAPPβ) by β-secretase cleavage corresponds to Aβ production accordingly, so it is desired to measure sAPPβ in parallel with Aβ. In addition, it is reported that APP gene mutation exists in individuals who suffer earlyonset Alzheimer’s disease. Swedish mutation, one of the APP gene mutations, is a double mutation at positions -1 to -2 from the β-secretase cleavage site (Lys670→Asn and Met671→Leu) . And further, it is reported that Swedish mutation elevates Aβ40 and Aβ42 production, and that the mutation is utilized in establishment of transgenic mice. The measuring sAPPβ in Swedish type is useful for research of AD as well as in wild type. On the one hand, it is considered that in the metabolic pathway of APP, APP is first cleaved by α-secretase rather than β-secretase normally to produce soluble APPα (sAPPα) and subsequently P3 is cleaved from the remaining C-terminal fragment by γ-secretase. This kit can measure human sAPPα in samples .

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 24 h (4°C), 30 (4°C), 30 min (RT)
- Standard Range: 0.78 - 50 ng/ml
-Sensitivity analytical: 0.09 ng/ml
- Final Sample Volume: 100 µl
- Sample Type: serum, plasma (EDTA), CSF, cell culture supernatant
- Isotope / Substrate: TMB 450 nm
- Regulatory Status: RUO