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Products specifications
| Technology | ELISA |
| Sizes | 96 tests |
Description
Enzyme immunoassay for the qualitative determination of IgM-class antibodies against Rubella Virus in human serum or plasma.
INTENDED USE:The Rubella Virus IgM µ-capture ELISA is intended for the determination of IgM-class antibodies against Rubella Virus in human serum or plasma (citrate, heparin). For Research Use Only – Not for Use in Diagnostic Procedures.
GENERAL INFORMATION:The immunoenzymatic determination of specific IgM-class antibodies is based on the ELISA (Enzymelinked Immunosorbent Assay) µ-capture technique. Microtiterplates are coated with anti-human IgM-class antibodies to bind the corresponding antibodies of the sample. After washing the wells to remove all unbound sample material, horseradish peroxidase (HRP) labelled antigen is added. This antigen-conjugate binds to the captured specific IgM antibodies. In a second washing step unbound conjugate is removed. The immune complexes are visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific IgM antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA Microtiterplate reader.
KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h (37°C), 30 min (RT), 15 min (RT/dark)
- Standard Range: Cut-off
- Sensitivity analytical: diagn. 100 %
- Final Sample Volume: 100 µl
- Sample Type: serum, plasma (citrate, heparin)
- Sample Pretreatment: 1:101 diluted
- Isotope / Substrate: TMB 450 nm
- Regulatory Status: CE0483/RUO
INTENDED USE:The Rubella Virus IgM µ-capture ELISA is intended for the determination of IgM-class antibodies against Rubella Virus in human serum or plasma (citrate, heparin). For Research Use Only – Not for Use in Diagnostic Procedures.
GENERAL INFORMATION:The immunoenzymatic determination of specific IgM-class antibodies is based on the ELISA (Enzymelinked Immunosorbent Assay) µ-capture technique. Microtiterplates are coated with anti-human IgM-class antibodies to bind the corresponding antibodies of the sample. After washing the wells to remove all unbound sample material, horseradish peroxidase (HRP) labelled antigen is added. This antigen-conjugate binds to the captured specific IgM antibodies. In a second washing step unbound conjugate is removed. The immune complexes are visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific IgM antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA Microtiterplate reader.
KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h (37°C), 30 min (RT), 15 min (RT/dark)
- Standard Range: Cut-off
- Sensitivity analytical: diagn. 100 %
- Final Sample Volume: 100 µl
- Sample Type: serum, plasma (citrate, heparin)
- Sample Pretreatment: 1:101 diluted
- Isotope / Substrate: TMB 450 nm
- Regulatory Status: CE0483/RUO