Real Time PCR Detection Kit Leptospira "pathogenic"

The Real Time PCR detection Kit Leptospira "pathogenic" dtec-qPCR comprises a series of specific targeted reagents designed for Leptospira “pathogenic” detection by using qPCR.

GENERAL INFORMATION:Leptospira is a genus of flexible, spiral-shaped, Gram-negative spirochetes with internal flagella. It is widely distributed, predominantly inhabiting stagnant water reservoirs such as bogs, lakes, ponds, and puddles, excluding Antarctica. These bacteria naturally infect a variety of animals hosts including cattle, pigs, horses, dogs, and rodents. Human infection, known as leptospirosis, present symptoms ranging from high fever to gastrointestinal distress, caused by contact with contaminated water or soil containing the urine of infected animals. Guernier et al. (2018) provided a phylogenetic classification of Leptospira species into three clusters based on their pathogenic potential. Among these, L. interrogans, L. kirschneri, L. borgpetersenii, L. mayottensis, L. santarosai, L. noguchii, L. weilii, L. kmetyi, L. tipperaryensis, L. alexandreri, and L. alstonii were identified as pathogenic. Our assay targets a signature region to detect this pathogenic cluster. However, due to the dynamic nature of Leptospira, variations in pathogenic species may result in strains not detected by the assay.

KIT CHARACTERISTICS:
- Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
- Resuspension buffer
- DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control