Real Time PCR Detection Kit Escherichia coli F18 (fedA)

The Real Time PCR detection Kit Escherichia coli F18 (fedA) comprises a series of specific targeted reagents designed for Escherichia coli F18 (fedA) detection by using qPCR.

GENERAL INFORMATION:Escherichia coli bacteria typically reside in the intestines of humans and animals. Most E. coli strains are harmless and play an essential role in maintaining a healthy human intestinal tract. However, some strains are pathogenic, meaning they can cause illness, including diarrhoea or infections outside the intestinal tract. The types of E. coli that can cause diarrhoea may be transmitted through contaminated food or water, or via contact with infected animals or humans. Among the pathogenic strains, enterotoxigenic E. coli (ETEC) is known to be a significant cause of diarrhoea, particularly in children and travelers. One of the most commonly detected types of fimbriae associated with ETEC is F18, encoded by the fedA gene. The intestinal adhesion and subsequent colonization by ETEC depend on specific receptors for fimbriae such as F4 or F18, which are essential for the bacteria to establish infection and cause disease.

KIT CHARACTERISTICS:
- Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
- Resuspension buffer
- DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control