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Products specifications
| Technology | qPCR / Real Time PCR/ PCR |
| Sizes | 4 targets: 4 x 100 reactions - FAM 4 targets + IC: 4 x 100 reactions - FAM, HEX Monodose 4 targets: 4 x 96 qPCR tubes - FAM Monodose 4 targets + IC: 4 x 96 qPCR tubes - FAM, HEX |
Description
Background:The RT-qPCR Panel comprises a series of specifically targeted reagents designed for Hemagglutinin typing of Avian influenza A virus H5, H7, H9 and N1 detection by using qPCR.
Influenza A viruses are negative-sense, single-stranded, segmented RNA viruses belonging to the Influenzavirus A genus in the Orthomyxoviridae family. Influenza A virus causes influenza in birds and some mammals. Despite strains of all subtypes having been uncommonly isolated from wild birds, some isolates cause severe disease both in domestic poultry and, rarely, in humans. The several subtypes of the Influenza A virus are labeled according to an H number (for the type of hemagglutinin) and an N number (for the type of neuraminidase).
There are 17 different H antigens (H1 to H17) and nine different N antigens (N1 to N9). Influenza A is generally recognized as Avian influenza, sometimes called avian flu, and colloquially, bird flu. Subtype H5 is detected worldwide in wild birds and poultry and usually are low pathogenic virus. Occasionally highly pathogenic viruses or human infections are detected, such as with Asian-origin highly pathogenic avian influenza A (H5N1) viruses. Avian influenza virus type A subtype H7 infection in humans is uncommon but has been documented in persons who have direct contact with infected birds. Illness in humans may include conjunctivitis and/or upper respiratory tract symptoms. Depending on the H7 strain, illness could be mild to moderate or severe to fatal. Avian influenza virus type A subtype H9 is documented only in a low pathogenic form in humans, but continuously circulates in poultry flocks causing enormous economic losses to the poultry industry.
Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control
Influenza A viruses are negative-sense, single-stranded, segmented RNA viruses belonging to the Influenzavirus A genus in the Orthomyxoviridae family. Influenza A virus causes influenza in birds and some mammals. Despite strains of all subtypes having been uncommonly isolated from wild birds, some isolates cause severe disease both in domestic poultry and, rarely, in humans. The several subtypes of the Influenza A virus are labeled according to an H number (for the type of hemagglutinin) and an N number (for the type of neuraminidase).
There are 17 different H antigens (H1 to H17) and nine different N antigens (N1 to N9). Influenza A is generally recognized as Avian influenza, sometimes called avian flu, and colloquially, bird flu. Subtype H5 is detected worldwide in wild birds and poultry and usually are low pathogenic virus. Occasionally highly pathogenic viruses or human infections are detected, such as with Asian-origin highly pathogenic avian influenza A (H5N1) viruses. Avian influenza virus type A subtype H7 infection in humans is uncommon but has been documented in persons who have direct contact with infected birds. Illness in humans may include conjunctivitis and/or upper respiratory tract symptoms. Depending on the H7 strain, illness could be mild to moderate or severe to fatal. Avian influenza virus type A subtype H9 is documented only in a low pathogenic form in humans, but continuously circulates in poultry flocks causing enormous economic losses to the poultry industry.
Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control
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