Real Time PCR Detection Kit Avian influenza type A virus subtypes H and N Panel (H5 and N9)

The Real Time PCR detection Kit Avian influenza type A virus subtypes H and N Panel (H5 and N9) comprises a series of specific targeted reagents designed for Avian influenza type A virus subtypes H5 and N9 detection by using qPCR.

GENERAL INFORMATION:Influenza A virus causes influenza in birds and some mammals. Influenzavirus A is a genus of the Orthomyxoviridae family. Strains of all subtypes of influenza A virus have been isolated from wild birds, although disease is uncommon. Some isolates of influenza A virus cause severe disease both in domestic poultry and, rarely, in humans. Influenza A viruses are negative-sense, single-stranded, segmented RNA viruses. The several subtypes are labelled according to an H number (hemagglutinin) and an N number (neuraminidase). Influenza A is generally recognized as Avian influenza, sometimes called avian flu, and colloquially, bird flu. Subtype H5 is detected worldwide in wild birds and poultry, and usually are low pathogenic viruses. Occasionally highly pathogenic viruses or human infections are detected, such as with Asian-origin highly pathogenic avian influenza A (H5N1) viruses. Despite the low pathogenic profile of H5N9, recently has been isolated an H5N9 highly pathogenic virus. This novel H5N9 subtype avian influenza A virus is a reassortant strain originating from H5N1, H7N9 and H9N2 subtypes, containing a characteristic motif of highly pathogenic strains. The assay was designed to cover sequences from the core cluster of the subtype. However, as Influenza virus are fast-evolving microorganisms, the inclusivity cannot be ensured for some new clonal strains of the virus.

KIT CHARACTERISTICS:
- Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
- Resuspension buffer
- DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control